Fig. 4: Agrin is necessary for amyloid-driven mechanoresponse. | Cell Death & Disease

Fig. 4: Agrin is necessary for amyloid-driven mechanoresponse.

From: Unveiling the mechanistic link between extracellular amyloid fibrils, mechano-signaling and YAP activation in cancer

Fig. 4

A Confocal fluorescence images of anti-YAP antibody (green) and DAPI staining (blue) in wild type (WT) IGR37 (siNC) cells upon treatment with 3I alone or in combination with rPMEL amyloid fibrils for 48 h, and of IGR37 Agrin KD cells (siAGRN) upon treatment with 3I alone or in combination with rPMEL amyloid fibrils for 48 h. Scale bar: 10 µm. B Nuclear/cytosol ratio of WT IGR37 (siNC) cells upon treatment with 3I alone or in combination with rPMEL amyloid fibrils for 48 h, and of IGR37 Agrin KD cells (siAGRN) upon treatment with 3I alone or in combination with rPMEL amyloid fibrils for 48 h. N = 15 replicates. T-test analysis, ****P < 0.0001. Data are presented as mean ± SD. C CTGF mRNA fold change in IGR39 WT (siNC) or Agrin KD cells (siAGRN) treated or not with rPMEL amyloid fibrils. N = 4 biological replicates. T-test analysis: ***P < 0.001, ****P < 0.0001. Data are presented as mean ± SD. D CYR61 mRNA fold change in IGR39 WT (siNC) or Agrin KD cells (siAGRN) treated or not with rPMEL amyloid fibrils. N = 3 biological replicates. T-test analysis: *P < 0.05, **P < 0.01. Data are presented as mean ± SD. E CTGF mRNA fold change in WT BXPC3 (siNC) or Agrin KD cells (siAGRN) treated with DMSO or 3I, or 3I and recombinant Aβ40. N = 3 biological replicates. T-test analysis: *P < 0.05, **P < 0.01. Data are presented as mean ± SD. F CYR61 mRNA fold change in WT BXPC3 (siNC) or Agrin KD cells (siAGRN) treated with DMSO or 3I, or 3I and recombinant Aβ40. N = 3 biological replicates. T-test analysis: *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as mean ± SD.

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