Fig. 5: LATS1 blocks amyloid-driven mechanotransduction. | Cell Death & Disease

Fig. 5: LATS1 blocks amyloid-driven mechanotransduction.

From: Unveiling the mechanistic link between extracellular amyloid fibrils, mechano-signaling and YAP activation in cancer

Fig. 5

A Representative cartoon of ROCK inhibition in the analyzed signaling pathway. B CTGF mRNA level in IGR37 cells treated with DMSO, ROCK inhibitor, ROCK inhibitor and 3I, or ROCK inhibitor, 3I and rPMEL amyloid fibrils. T-test analysis: *P < 0.05, ***P < 0.001. Data are presented as mean ± SD. C CYR61 mRNA level in IGR37 cells treated with DMSO, ROCK inhibitor, ROCK inhibitor and 3I, or ROCK inhibitor, 3I and rPMEL amyloid fibrils. T-test analysis: *P < 0.05, ***P < 0.001. Data are presented as mean ± SD. D CTGF mRNA level in IGR39 cells treated with rPMEL amyloid fibrils, ROCK inhibitor, or ROCK inhibitor and rPMEL amyloid fibrils. T-test analysis: **P < 0.01, ****P < 0.0001. Data are presented as mean ± SD. E CYR61 mRNA level in IGR39 cells treated with rPMEL amyloid fibrils, ROCK inhibitor, or ROCK inhibitor and rPMEL amyloid fibrils. T-test analysis: *P < 0.05, ***P < 0.001. Data are presented as mean ± SD. F Representative cartoon of LATS1 overexpression in the analyzed signaling pathway. G CTGF mRNA level in IGR37 WT (GFP) and IGR37 cells overexpressing LATS1 (LATS1OE) treated with DMSO or 3I, or 3I and rPMEL amyloid fibrils (3 l+PMEL). T-test analysis: ns P > 0.05, *P < 0.05, **P < 0.01. Data are presented as mean ± SD. H CYR61 mRNA level in IGR37 WT (GFP) and IGR37 cells overexpressing LATS1 (LATS1OE) treated with DMSO or 3I, or 3I and rPMEL amyloid fibrils (3 l+PMEL). T-test analysis: ns P > 0.05, *P < 0.05, **P < 0.01. Data are presented as mean ± SD. I CTGF mRNA level in IGR39 WT (GFP) or LATS1 overexpressing cells (LATS1OE) treated or not with rPMEL amyloid fibrils. ns P > 0.05, ***P < 0.001. Data are presented as mean ± SD. J CYR61 mRNA level in IGR39 WT (GFP) or LATS1 overexpressing cells (LATS1OE) treated or not with rPMEL amyloid fibrils. ns P > 0.05, **P < 0.01. Data are presented as mean ± SD.

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