Fig. 1: N-glycan profiles in mammary carcinoma cell lines with different HA-producing abilities. | Cell Death & Disease

Fig. 1: N-glycan profiles in mammary carcinoma cell lines with different HA-producing abilities.

From: Tolerable glycometabolic stress boosts cancer cell resilience through altered N-glycosylation and Notch signaling activation

Fig. 1

A Typical MALDI-TOF MS spectra of N-glycans in HA-high Has2ΔNeo cells. The estimated N-glycan structures are shown. IS, internal standard. B N-glycan composition of parental Neu, HA-low Has2+Neo, and HA-high Has2ΔNeo cells. The N-glycan composition was expressed as the ratio of each glycan type to the total N-glycans. C Relative amounts of PM, HM, and C/H-type N-glycans. The colors representing each glycoform are listed in Supplementary Table S1. Data are the mean ± SD from n = 3. Two-tailed Student’s t-test. *p < 0.05, **p < 0.01. D Heatmap analysis based on the quantitative glycomic profiles of N-glycans. The heatmap represents the normalized abundance of N-glycans in Neu, Has2+Neo, and Has2ΔNeo cells. The complete structures of the N-glycans are shown in Supplementary Table S1. The bars on the right side of the heatmap indicate modifications of the N-glycan. Selected glycan structures are shown on the right, with important modifications (red circles).

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