Table 1 PCR primers used in this study.
From: Hedgehog signalling is involved in acquired resistance to KRASG12C inhibitors in lung cancer cells
Primers |  | Sequences (5′-3′) | |
|---|---|---|---|
Gene expression analysis | ARL6 | F | GGCTTCAAGATCAGATCCAGACT |
R | AAGGTCAGAGTCCATAAATGCAAG | ||
β-actin | F | ATCGTGCGTGACATTAAGGAGAAG | |
R | AGGAAGGAAGGCTGCAAG | ||
AURKA | F | GCAACCAGTGTACCTCATCCTG | |
R | AAGTCTTCCAAAGCCCACTGCC | ||
GAPDH | F | AATCCCATCACCATCTTCCAG | |
R | AAATGAGCCCCAGCCTTC | ||
GLI1 | F | ACAGCCAGTGTCCTCGACTT | |
R | ATAGGGGCCTGACTGGAGAT | ||
IFT88 | F | GCCGAAGCACTTAACACTTAT | |
R | GTCTAATGCCATTCGGTAGAA | ||
KRAS | F | TCGACACAGCAGGTCAAGAG | |
R | CAAAGAAAGCCCTCCCCAGT | ||
PTCH1 | F | CTCCTTTGCGGTGGACAA | |
R | CCTCAGCCTTATTCAGCATTTC | ||
ChIP analysis | KRAS-P1 | F | CTAGGAGGGGGAGACTGGAA |
R | AGCGCCTGTACCTGATAGGA | ||
KRAS-P2 | F | TCCTATCAGGTACAGGCGCT | |
R | CTCCACAGAGAAGCTGCGAA | ||
KRAS-P3 | F | TAAGTCCCCGAAGTCGCCTC | |
R | CTGCCTAGCCGCAAGGCTGT | ||
KRAS-P4 | F | ACAGCCTTGCGGCTAGGCAG | |
R | AAATCGAGCTCCGAGCACACCG | ||
KRAS-P5 | F | GGAAAGGATGACAGTTGATGTAAAG | |
R | TCCGAGACTTTCAGTTCCATTC | ||
