Fig. 1: ZBTB7B deficiency accelerates Akt/N-Ras-induced liver cancer.

A Phylogenetic analysis of gene expression in the Zbtb7b^f/f and Alb-Cre, Zbtb7b^f/f (Zbtb7b^Δli) adult livers, and published gene expression profiles of adult and fetal livers. B GSEA comparison of liver-specific gene expression between the Zbtb7b^f/f and Zbtb7b^Δli livers. C GSEA comparison of liver cancer gene signatures between the Zbtb7b^f/f and Zbtb7b^Δli livers. D Study design. Hydrodynamic tail vein injection (HTVi) of Akt and N-Ras to induce tumor development in control (Alb-Cre or Zbtb7b^f/f, Ctrl) or Zbtb7b^Δli mice. E Kaplan–Meier survival analysis. Control (n = 6), Zbtb7b^Δli (n = 7). F Gross liver images of control and Zbtb7b^Δli mice 4 weeks after injection with vector (Vec) or Akt/N-Ras. G Liver/body weight ratio of control and Zbtb7b^Δli mice 4 weeks after injection with vector or Akt/N-Ras. n = 5. H H&E staining of liver sections of control and Zbtb7b^Δli mice 4 weeks after injection with vector or Akt/N-Ras. Magnification: ×4. Scale bar: 500 μm. I, J Numbers of tumors (I) and percentage of tumor area (J) in the livers of control and Zbtb7b^Δli mice 4 weeks after Akt/N-Ras injection. n = 5. K Immunohistochemistry of HA-tag and Ki67 on liver sections of control and Zbtb7b^Δli mice 4 weeks after Akt/N-Ras injection. Magnification: ×10. Scale bar: 200 μm. L Percentage of Ki67⁺ hepatocytes in the livers of control and Zbtb7b^Δli mice 4 weeks after Akt/N-Ras injection. n = 5. Data are presented as mean ± SEM. Statistical analyses were performed with the Log-Rank test (E), two-way ANOVA followed by Šídák’s multiple comparison tests (G) or two-tailed unpaired student’s t test (I, J, L). *P < 0.05, ***P < 0.001. ns: not significant.