Fig. 7: ZBTB7B attenuates the tumor-promoting functions of c-Jun. | Cell Death & Disease

Fig. 7: ZBTB7B attenuates the tumor-promoting functions of c-Jun.

From: ZBTB7B is a permissive regulator of hepatocellular carcinoma initiation by repressing c-Jun expression and function

Fig. 7

A Study design. Hydrodynamic tail vein injection (HTVi) of Akt and N-Ras oncogenes with active c-Jun (c-Jun S63D/S73D) and ZBTB7B. Mice were sacrificed 2 weeks or 4 weeks after hydrodynamic injection. n = 5. B H&E staining on liver sections of wild-type mice 2 weeks after Akt/N-Ras injection with active c-Jun and ZBTB7B. Magnification: ×10. Scale bar: 200 μm. C Numbers of tumor nodules 2 weeks after Akt/N-Ras injection with active c-Jun and ZBTB7B. D H&E staining and immunohistochemistry of HA-tag and Ki67 on liver sections of wild-type mice 4 weeks after Akt/N-Ras injection with active c-Jun and ZBTB7B. Magnification: ×10. Scale bars: 200 μm. E–G Numbers of tumors (E), percentage of tumor area (F), and Ki67+ hepatocytes (G) in the livers 4 weeks after Akt/N-Ras injection with active c-Jun and ZBTB7B. Data are presented as mean ± SEM. Statistical analyses were performed with one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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