Fig. 4: IMT1 is ineffective in POLRMT-silenced OS cells. | Cell Death & Disease

Fig. 4: IMT1 is ineffective in POLRMT-silenced OS cells.

From: Targeting POLRMT by a first-in-class inhibitor IMT1 inhibits osteosarcoma cell growth in vitro and in vivo

Fig. 4

The stable primary pOS-1 cells with the lentiviral POLRMT shRNA (“shPOLRMT”) were treated with or without IMT1 (1 μM) for indicated time periods, control cells with the scramble control shRNA (“shC”) were left untreated. Expression of listed mRNAs and proteins was shown (A–C); Cell proliferation (EdU dye assay D) and in vitro migration (“Transwell” assay, E) were measured via the corresponding methods, with results quantified; Mitochondrial depolarization was analyzed by JC-1 staining (F); ROS production was tested via CellROX dye assay (G), with cell apoptosis examined by TUNEL-nuclei staining assay (H). *P < 0.05 indicates a significant difference compared to the “shC” treatment. “N. S.” represents P > 0.05, indicating no statistical difference. The data is presented as mean ± SD. The experiments presented in this figure were replicated five times (n = 5, with five biological replicates), yielding consistent and similar results. The scale bar is set at 100 μm.

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