Fig. 4: USP43 interacts with c-Myc.
From: USP43 stabilizes c-Myc to promote glycolysis and metastasis in bladder cancer
A, B Co-IP assay showed that exogenous USP43 interacted with c-Myc in 293 T cells. USP43 and c-Myc were precipitated with the corresponding anti-Flag (A) and anti-HA antibodies (B), respectively. C Recombinant purified GST-tagged c-Myc protein and His-tagged USP43 protein from E. coli were subjected to a GST pull-down assay in vitro. D, E Schematic diagram of c-Myc (D) and USP43 (E) truncations. F, H Flag-USP43 was cotransfected with c-Myc truncation mutants. Interactions were analyzed using the Co-IP assay. G HA-c-Myc was cotransfected with USP43 truncation mutants. Interactions were analyzed using the Co-IP assay. I c-Myc in T24 cell lysates was precipitated by c-Myc antibody. The interaction between endogenous USP43 and c-Myc was examined by Western blot analysis. J USP43 and c-Myc were transfected into UM-UC-3 cells for immunofluorescence assays. Colocalization analysis showed that USP43 and c-Myc colocalized in the nucleus. The scale bar is 10 μm.
