Fig. 2: RNA-seq analysis of manipulated T-ALL cells.
A Visualization of BACH2 transcriptional levels (red peaks) with IGV from two replicates in each group. GRCh38 genome was used as a human reference genome (upper). Volcano plot was generated with 137 Up-DEGs and 96 Down-DEGs in BACH2OE Jurkat cells vs. control cells (lower). The Log2(FC), adjusted p values were calculated using DESeq2 default settings, and significant genes were considered the ones with adjusted p values < 0.05. B Heatmap of the top 20 Up- and Down-DEGs from two replicates in each group. The level of gene expression is indicated by the color intensity, with red representing high expression and blue representing low expression. C Top 10 of GO terms enriched in BP category based on the enrichment analysis of the obtained DEGs. D DEGs that were enriched in the top 5 GO-BP terms. E Top 15 hub genes in BACH2-involving gene regulatory network using weighted gene co-expression network analysis. The black arrows pointed to the downstream target genes and the lines indicated the gene-gene interactions. Red, green, and blue represent the Up-DEGs (n = 5), Down-DEGs (n = 4), and nonsignificant genes (n = 6), respectively. F Top 12 hub nodes in BACH2-involving PPI network, with red representing the Up-DEGs (n = 8) and blue representing the Down-DEGs (n = 4).
