Fig. 3: High-LCFAs diet exacerbates intestinal barrier damage in DSS-induced colitis mice. | Cell Death & Disease

Fig. 3: High-LCFAs diet exacerbates intestinal barrier damage in DSS-induced colitis mice.

From: Dietary long-chain fatty acids promote colitis by regulating palmitoylation of STAT3 through CD36-mediated endocytosis

Fig. 3

A The remaining fluorescence and absorption of FITC-dextran. Red represents the highest intensity, while dark purple indicates the lowest intensity; B The plasmatic concentration of FITC-dextran (n = 6); C Levels of IL-6, TNF-α, and IL-1β in colonic tissues of mice (n = 6); D Representative images of colonic tissue IHC staining for ZO-1; E Representative images of colonic tissue IF staining for ZO-1; F Scanning electron microscopy images of mouse colonic tissue sections, with magnified regions showing tight junctions; G Representative images of colonic tissue staining with AB-PAS and IHC of MUC-2; H TUNEL staining of colonic tissue; I Statistical analysis of TUNEL-positive cells; J Western blotting of colonic tissues. In vitro experiments: NCM460 cells stimulated with 1ug mL−1 LPS in combination with 200 µM PA, 100 µM SA, or BSA for 24 h; K ELISA measurement of IL-6 levels in cell culture supernatants; L Western blotting of cells; M Representative images of IF staining of ZO-1 IF in NCM460 cell. Statistical significance was determined using one-way ANOVA test. ***p < 0.001, **p < 0.01, *p < 0.05.

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