Fig. 6: IL-17 regulates osteoclast energy metabolism via Glu.
From: IL-17 promotes osteoclast-induced bone loss by regulating glutamine-dependent energy metabolism
BMDMs were cultured with the medium that containing M-CSF (30 ng/ml) and RANKL (75 ng/mL) for 5 days with or without Glu deprivation, as well as treated with indicated stimulation, including IL-17(0.1 ng/ml), V9302 (5 μM) and α-KG (0.5 mM). A, B BMDMs were seeded in Seahorse XF analyzer culture plates and treated as described. Extracellular acidification rate (ECAR) was analyzed by XF Cell Mito Stress Assay. C, D BMDMs were seeded in Seahorse XFp analyzer culture plates and treated as described. Oxygen consumption rate (OCR) were analyzed by XF Cell Mito Stress Assay. E BMDMs were seeded in 6 well plates and treated as described. The levels of lactate were analyzed through Lactate Assay kit. F, G BMDMs were cultured with the Glu deprived medium that containing M-CSF (30 ng/ml) and RANKL (75 ng/mL) for 5 days, as well as treated with or without IL-17 (0.1 ng/ml) and α-KG (0.5 mM). TRAP-positive multinucleated (>3 nuclei) cells were counted as osteoclasts. H, I QPCR and Western blot assay examining the the expression of gene of osteoclast marker and IL-17 signaling pathway. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significance.
