Fig. 7: Cuproptosis mechanisms are shared in the model of copper homeostasis dysregulation. | Cell Death & Disease

Fig. 7: Cuproptosis mechanisms are shared in the model of copper homeostasis dysregulation.

From: Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers

Fig. 7

A Protein level of SLC31A1 was assessed by western blot. B Protein content was analyzed and SLC31A1 overexpression 293 T cells 24 hours after treatment of supplementation with indicated concentrations of CuCl2. C Flow cytometry assay of ROS. The cells were treated with or without 4 μM CuCl2 for 24 hours. D RT-qPCR was used to determine expression of HSPA9 at indicated treatment. E, F Viability was measured by CCK-8 (C) and EDU (D) in 293 T cells and SLC31A1 overexpression 293 T cells under indicated treatment. G Protein content was analyzed and SLC31A1 overexpression 293 T cells with indicated treatment for 24 hours. H, I Cell cycle of SLC31A1 overexpression 293 T cells 24 hours after treatment of supplementation with or without CuCl2. J Protein content was analyzed in SLC31A1 overexpression 293 T cells 24 hours after indicated treatment. For treatment of TTM and GBZ, the cells were treated with 10 μM TTM or 20 μM GBZ for 24 hours and then collected. K ChlP-qPCR for H3K4me1, H3K4me2, H3K4me3 and IgG control in SLC31A1 overexpression 293 T cells 24 hours after treatment of supplementation with or without CuCl2. Data are presented as the means ± SD from independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.

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