Fig. 6: MTX augmented the anti-tumor effect of JQ1 in NB.
From: MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma
A Proliferation of SK-N-BE(2) cells treated with vehicle control (Ctrl), 0.2 μM JQ1, 0.2 μM MTX, 0.2 μM JQ1 + 0.2 μM MTX. B Proliferation of IMR32 cells treated with vehicle control (Ctrl), 0.2 μM JQ1, 0.2 μM MTX, 0.2 μM JQ1 + 0.2 μM MTX. C Changes of apoptosis rates in SK-N-BE(2) and IMR32 cells treated with vehicle control, 0.2 μM MTX or 0.2 μM JQ1 for 72 h. D Pictures of subcutaneous xenografts. E Growth curves of subcutaneous xenografts administered with vehicle control (Ctrl), 50 mg/kg/d JQ1 and/or twice weekly with MTX 20 mg/kg/d. F Quantification of tumor mass. G The graphic illustration of regulatory mechanism diagram of our study. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
