Fig. 3: ID4 controls FA formation by stimulating the production and release of VEGFA in BC cells.

A cytokine array analysis performed with conditioned medium from M6 Control and ID4-KO cells. B western blot analysis of VEGFA in conditioned medium from M6 Control and ID4-KO cells with the relative quantification graph. Ponceau S was used for protein normalization. C real-time PCR analysis of VEGFA expression in M6 Control and ID4-KO cells. D western blot analysis of p-FAK and FAK in M6 Control, ID4-KO, and ID4-KO cells treated with recombinant VEGFA for 24 h, with the quantification graph. E immunofluorescence staining of vinculin in M6 Control, ID4-KO, and ID4-KO cells treated with VEGFA for 24 h, and graph showing the quantification of the vinculin-positive area. Scale bar: 20 µm. Data are presented as mean ± SD. **P < 0.01, **** P < 0.0001 calculated by One-way Anova test on n = 3 experiments.