Fig. 4: ID4-mediated VEGFA production promotes integrin β3 and VEGFR2 interaction in BC cells.

A Immunofluorescence staining of integrin β3 and integrin β1 in M6 Control and ID4-KO cells. B western blot analysis of p-integrin β3 and integrin β3 in M6 Control and ID4-KO cells, with the relative quantification graph. C PLA analysis of integrin β3 and VEGFR2 interaction in M6 Control, ID4-KO, and ID4-KO cells treated with VEGFA for 24 h, with the relative quantification graph. D PLA analysis of integrin β3 and VEGFR2 interaction in MDA-MB-468 Control, ID4-KO, and ID4-KO cells treated with VEGFA for 24 h, with the relative quantification graph. E transmigration experiment of M6 Control and ID4-KO cells treated with Cilengitide and VEGFA alone or in combination, and quantification of migrated cells at 24 h. F transmigration experiment of MDA-MB-468 Control and ID4-KO cells treated with Cilengitide and VEGFA alone or in combination, and quantification of migrated cells at 24 h. Scale bar: 20 µm. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001 calculated by Student’s t test (B, E, F) or One-way Anova test (C, D, E, F) on n = 3 experiments.