Fig. 5: ID4-KO BC cells display reduced YAP/TAZ nuclear localization and activation.

A immunofluorescence analysis of YAP in M6 Control and ID4-KO cells. B immunofluorescence analysis of TAZ in M6 Control and ID4-KO cells. C quantification of YAP mean fluorescence intensity in the nuclei and cytoplasm of M6 Control and ID4-KO cells. D quantification of TAZ mean fluorescence intensity in the nuclei and cytoplasm of M6 Control and ID4-KO cells. E real-time analysis of TEAD1 and CTGF in M6 Control and ID4-KO cells grown in peptigel alpha 4. F real-time analysis of TEAD1 and CTGF in M6 Control and ID4-KO cells grown in peptigel gamma 4. Scale bar: 20 µm. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 calculated by One-way Anova (C, D) or Student’s t test (E, F) on n = 3 experiments.