Fig. 3: Knockdown of ATMIN inhibits cell proliferation and facilitates docetaxel-sensitivity of NPC cells in vitro.
From: Transcription factor ATMIN facilitates chemoresistance in nasopharyngeal carcinoma
A, B qRT-PCR and western blot analysis of ATMIN expression in HONE1 and SUNE1 cells transfected with si-ATMIN#1 or si-ATMIN#2 or si-NC. C CCK-8 assays determining the growth curves of the transfected cells. D Representative images and quantification of the colony formation assays in the transfected cells. E CCK-8 assays testing the sensitivity of the transfected cells to the indicated doses of DTX, DDP and 5-FU. F qRT-PCR and western blot analysis of ATMIN expression in HONE1 and SUNE1 cells transfected with FLAG-ATMIN. G CCK-8 assays determining the growth curves of the SUNE1 and HONE1 cells transfected with vector or FLAG-ATMIN. H Representative images and quantification of the colony formation assays in the SUNE1 and HONE1 cells transfected with vector or FLAG-ATMIN. I CCK-8 assays testing the sensitivity of the SUNE1 and HONE1 cells transfected with vector or FLAG-ATMIN to the indicated doses of DTX. Data are presented as mean ± SD, P values were calculated using one-way ANOVA (A, C–E) or Student’s t test (F–I). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns not significant. DTX docetaxel, DDP cis-diamminedichloro-platinum, 5-FU 5-fluorouracil. The unprocessed images of the blots are shown in Supplementary Fig. S3.
