Fig. 4: CUL4B deficiency enhances synapse formation and neuronal excitability.
From: CUL4B mutations impair human cortical neurogenesis through PP2A-dependent inhibition of AKT and ERK
A Representative images of sections from the day-60 cerebral organoids stained with SYN1 and MAP2. Scale bar, 20 μm. B Quantification of SYN1+ puncta density in both the isogenic control (Correct) and the patient-derived (Patient) organoids on day 60. N = 12. C Representative bright-field image of a day-45 organoid plated on a 64-electrode array in an MEA well. D Plate maps of mean firing rates of both Correct and Patient organoids on day 45 and day 60. E Representative wave forms of spiking behavior from a single electrode for each Correct or Patient organoid on day 60. F Representative raster plots of Correct and Patient organoids (day 60) over 5-min continuous recording. Quantification of mean firing rate (G), number of spikes (H), burst percentage (I) and burst frequency (J) based on data obtained from Correct and Patient organoids on day 45 and day 60 over 10 min recording. A bundle of more than 10 spikes within 100 ms was defined as a burst. N = 8 MEA wells per genotype. Data are presented as the mean ± SEM. The statistical significance was determined using two-tailed unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.001. ns: no significance.
