Fig. 8: Acetylation of GSDMD promotes its ubiquitination.
From: Crosstalk of HDAC4, PP1, and GSDMD in controlling pyroptosis
A IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Flag-GSDMD-p30 and HA-GSDMD-p30, together with or without Myc-HDAC4 or TSA. B IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Myc-HDAC4 and Flag-GSDMD or its mutants. C IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Flag-GSDMD and HA-UB, treat with or without TSA. D IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Flag-GSDMD and HA-UB, together with HDAC4 or its mutants. E NC and sg-HDAC4 HEK293T cells were transfected with Flag-GSDMD, together with or without Myc-HDAC4. GSDMD ubiquitination was analyzed by immunoprecipitation with an anti-GSDMD antibody followed by western blotting. IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Flag-GSDMD and HA-UB (F) or HA-K27 (G). All results shown are representative of at least three independent experiments. H IB of total cell lysates (input) and proteins immunoprecipitated with anti-Flag resin from HEK293T cells transfected with Flag-GSDMD or Flag-GSDMD-K248R and HA-K6/ K11/ K29/ K33/ K48/ K63.
