Fig. 3: HD cells display increased cellular susceptibility to oxidative stress. | Cell Death & Disease

Fig. 3: HD cells display increased cellular susceptibility to oxidative stress.

From: CHCHD2 up-regulation in Huntington disease mediates a compensatory protective response against oxidative stress

Fig. 3

A Mitochondrial ROS in Q7 and Q111 cells under basal and TBHP treatment conditions are detected by MitoSox Red. B Cellular ROS in Q7 and Q111 cells under basal and TBHP treatment conditions are detected by H2DCFDA. n = 3 biological replicates for (A, B). C Q7 and Q111 cells were treated with different concentrations (0, 50, 100, 200, 400, and 800 µM) of TBHP for 24 h. Cell viability was determined by CellTiter-Glo assay. n = 8 biological replicates. D, E Cleaved Caspase-3 expression levels in Q7 and Q111 cells after 50 µM THBP treatment for 24 h were determined by immunoblotting. n = 3 independent biological replicates; Values shown as mean ± SEM. ns, no significance, **p < 0.01, ***p < 0.001, and ****p < 0.0001 for shown comparisons, and ###p < 0.001, and ####p < 0.0001 relative to the corresponding Q7 group was determined by two-way ANOVA analysis followed by a Bonferroni post hoc multiple-comparison test.

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