Fig. 5: IL-9 secreted by CLL cells promotes PD-1 expression in CTLs. | Cell Death & Disease

Fig. 5: IL-9 secreted by CLL cells promotes PD-1 expression in CTLs.

From: Leukemic cell-secreted interleukin-9 suppresses cytotoxic T cell-mediated killing in chronic lymphocytic leukemia

Fig. 5

A, B Flow cytometric analysis of surface PD-1 in CD8+ cells purified from buffy coats, stimulated with anti-CD3/CD28 mAb-coated beads + IL-2 for 48 h (CTLs), and cultured in conditioned media in the presence of either control (iso Ab), anti-IL-9 (IL-9 Ab, A) or anti-IL-10 (IL-10 Ab, B) antibodies and/or recombinant IL-9 (rIL-9) (A), or recombinant IL-10 (rIL-10) (B) (n buffy coats from healthy donors = 4; one-way ANOVA). C Correlation between surface PD-1 (MFI) in CD8+ cells isolated from CLL patients and IL-9 mRNA in leukemic cells isolated from the respective CLL patients (n = 18). D Spearman r correlation index between surface PD-1 (MFI) in CD8+ cells isolated from CLL patients and IL-9 and p66Shc mRNA in CLL cells from the respective CLL patients (n = 18). E Flow cytometric analysis of surface PD-1 in CD8+ cells isolated from peripheral blood of CLL patients, grouped in M-CLL (n = 10) and U-CLL (n = 8) patients according to the IGHV mutational status. Data are expressed as mean ± SD. ****p ≤ 0.0001; ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05.

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