Fig. 3: Sirt6 overexpression counteracted APAP-induced hepatocyte death.
From: Hepatic Sirt6 activation abrogates acute liver failure
WT and Sirt6-Tg mice were treated with APAP (500 mg/kg) for 8 h. Liver sections were prepared and analyzed for the markers of cell death. A TUNEL staining of the liver sections. scale bar corresponds to 50 μm, and results were presented as mean ± SEM (n = 6), **P < 0.01. B HMGB1 staining of liver sections, scale bar corresponds to 40 μm. C Liver GSH concentrations. Results were presented as mean ± SEM (n = 6), **P < 0.01. D Western blotting of Sirt6 in mouse hepatocytes isolated from WT mice and Sirt6-Tg mice. E, F TUNEL assay of cell death of WT and Sirt6-Tg mouse hepatocytes treated with APAP (10 mM) or PBS for 8 h. Scale bar corresponds to 10 μm. Results were presented as mean ± SEM (n = 3–4), **P < 0.01. Statistical analysis was performed by unpaired Student’s t test (A, C), and two-way ANOVA plus Bonferroni’s multiple comparisons test (F).
