Fig. 4: Sirt6 overexpression reduced APAP-induced liver necrosis and apoptosis.

A The production of ROS in vivo was measured by DHE staining in the liver sections of WT and Sirt6-Tg mice treated with APAP (500 mg/kg) for 8 h (scale bar corresponds to 50 μm). Results were presented as mean ± SEM (n = 3), ^*P < 0.05. B JNK activation by Western blotting in liver extracts from WT and Sirt6-Tg mice treated with APAP (500 mg/kg) or saline for the indicated times. Blots were quantified and results were presented as mean ± SEM (n = 5 for each genotype at each time point), ^**P < 0.01 compared with the WT + APAP group. C JNK activation analyzed by Western blotting in WT and Sirt6-Tg mouse hepatocytes treated with APAP (10 mM) or PBS for 8 h. Blots were quantified and results were presented as mean ± SEM (n = 3), ^*P < 0.05. D Western blotting of indicated proteins in the liver tissues from WT and Sirt6-Tg mice treated with APAP (500 mg/kg) for 8 h. Blots were quantified and results were presented as mean ± SEM (n = 3), ^*P < 0.05. E Western blotting of Nrf2, HO-1 in the liver tissues from WT and Sirt6-Tg mice treated with APAP (500 mg/kg) for 8 h. Blots were quantified and results were presented as mean ± SEM (n = 3), ^**P < 0.01. F Nrf2 nuclear translocation in the liver tissues from WT and Sirt6-Tg mice treated with APAP (500 mg/kg) for 8 h. Blots were quantified and results were presented as mean ± SEM (n = 3), *P < 0.05. Statistical analysis was performed by unpaired Student’s t test (A, D, E, F), and two-way ANOVA plus Bonferroni’s multiple comparisons test (B, C).