Fig. 7: Overexpression of hepatic Sirt6 attenuated APAP-induced hepatotoxicity.

A Schematic diagram for the generation of Sirt6-HepTg mice. B Western blotting analysis of Sirt6 in the liver, lung, spleen, and kidney from Sirt6-HepTg mice. α-Tubulin was detected as a loading control. C Serum ALT (left) and AST (right) activity in Control and Sirt6-HepTg mice treated with APAP (500 mg/kg) for 8 h. Each dot or triangle symbol represents a different mouse sample. Results were presented as mean ± SEM (n = 6), ^*P < 0.05. D H&E staining of liver sections from Control and Sirt6-HepTg mice treated with APAP (500 mg/kg) for 8 h. 10×, scale bar corresponds to 200 µm. Quantification of necrotic areas by ImageJ software. Results were presented as mean ± SEM (n = 6), ^**P < 0.01. E Survival rate of Control and Sirt6-HepTg mice treated with APAP (750 mg/kg) for 72 h (n = 7), ^*P < 0.05. F JNK activation analyzed by Western blotting in the liver tissues from Control and Sirt6-HepTg mice treated with APAP (500 mg/kg) for 8 h. Blots were quantified and results were presented as mean ± SEM (n = 3), ^*P < 0.05. Statistical analysis was performed by unpaired Student’s t test (C, D, F), and log-rank test (E).