Fig. 2: Myeloid-specific TSC1 deficiency exacerbates hepatocellular damage in I/R-induced liver injury. | Cell Death & Disease

Fig. 2: Myeloid-specific TSC1 deficiency exacerbates hepatocellular damage in I/R-induced liver injury.

From: Loss of macrophage TSC1 exacerbates sterile inflammatory liver injury through inhibiting the AKT/MST1/NRF2 signaling pathway

Fig. 2

A Western-blot analysis of TSC1, p-MST1 and MST1 protein expression in liver macrophages from I/R-stressed livers from mice subjected to 90 min of partial liver warm ischemia, followed by 6 h, 12 h or 24 h of reperfusion. B Western-blot analysis of TSC1 in macrophages during I/R. C Mice were subjected to 90 min of partial liver warm ischemia, followed by 6 h of reperfusion. Representative histological staining (H&E) of ischemic liver tissue (n = 4–6/group), Scale bars = 250 μm. D Liver damage, evaluated by Suzuki’s score. ***p < 0.001. E Hepatocellular function, as assessed by serum ALT levels (IU/L). The results are expressed as the Mean ± SD (n = 4–6 samples/group). ***p < 0.001. F Liver neutrophil accumulation, as determined by MPO activity (U/g). The results are expressed as the Mean ± SD (representative of 4–6 mice/group). **p < 0.01. G Quantitation of ROS-sensing dye DHE staining, MDA, and GSH activities were assessed in ischemic liver tissues. *p < 0.05, **p < 0.01.

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