Fig. 1: AOPP inhibited autophagy and lysosomal biogenesis in granulosa cells. | Cell Death & Disease

Fig. 1: AOPP inhibited autophagy and lysosomal biogenesis in granulosa cells.

From: Advanced oxidation protein products attenuate the autophagy-lysosome pathway in ovarian granulosa cells by modulating the ROS-dependent mTOR-TFEB pathway

Fig. 1

A Representative blots and quantification showing p62 and LC3B II expression levels in negative control and KGN/COV434 cells treated with 200 μg/mL BSA, 50 μg/mL AOPPs, 100 μg/mL AOPPs, and 200 μg/mL AOPPs for 48 h. B Representative blots and quantification showing p62 and LC3B II expression levels in negative control, KGN/COV434 cells treated with 200 μg/mL AOPPs for 48 h (AOPP group), KGN/COV434 cells treated with 30 μM CQ for 4 h (CQ group), and KGN/COV434 cells pretreated with AOPPs for 48 h and then treated with CQ for 4 h (CQ + AOPP group). C Representative blots and quantification showing p62 and LC3B II expression levels in negative control, KGN/COV434 cells treated with 200 μg/mL AOPPs for 48 h (AOPP group), KGN/COV434 cells treated with 100 nM nM Baf A1 for 4 h (Baf A1 group), and KGN/COV434 cells pretreated with AOPPs for 48 h and then treated with Baf A1 for 4 h (Baf A1 + AOPP group). D Ultrastructural analysis of autophagosomes in negative control and KGN/COV434 cells treated with 200 μg/mL BSA (the BSA group) and 200 μg/mL AOPPs for 48 h (the AOPP group). The arrows indicate autophagosomes. Scale bars: 2 μm. E Representative images of RFP-GFP-LC3 under a confocal laser scanning microscope in control group, BSA group, AOPP group, CQ group, and CQ + AOPP group. The GFP + RFP+ (yellow) puncta are autophagosomes. The numbers of autophagosomes were quantified for 10 cells. High-magnification images of the indicated boxed areas are shown in the rightmost panels. F Lysosomal biogenesis assessed by Lyso-Tracker Red in control group, BSA group, and AOPP group. G LAMP1 expression as detected by immunofluorescence in control group, BSA group, and AOPP group. All experiments were repeated for three times. The results are expressed as the mean ± SD from experiments performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.001. Statistical significance was determined by one-way ANOVA and Tukey tests for post hoc comparisons.

Back to article page