Fig. 1: Pyroptosis key gene GSDME correlates with psoriasis.

A 812 normal skin tissues from GTEx database were classified into “High GSDME” group (30 cases) and “Low GSDME” group (30 cases) based on Reads Per Kilobase per Million mapped reads (RPKMs) of GSDME. B Heat map from hierarchical cluster analysis revealed the differentially expressed genes (DEGs) between two groups were enriched in keratinocyte functions. C GSEA analysis enriched GO BP plots, including skin development and keratinocyte differentiation, which were positively correlated with the “Low GSDME” group. D Comparison of GSDME mRNA RPKMs among normal skin from healthy controls (NN), non-lesional skin from patients (PN) and lesional skin from patients (PP) using datasets GSE13355, GSE14905, and GSE30999. E In keratinocytes, GSDME expression was higher in lesions of psoriasis patients compared to that of healthy people, from scRNA-seq data of GSE162183. F Correlation between GSDME level and psoriatic indicators (IL-17A mRNA level) or PASI scores in psoriasis patients were analyzed by Pearson or Spearman correlation analysis from GSE14905 and GSE85034. G, H The changes of GSDME mRNA level in lesional skin tissues of psoriasis patients (from GSE53552 and GSE85034) after treatment with Brodalumab or Adalimumab. BP biological process, CC cellular component, MF molecular function, NES Normalized Enrichment Score, FDR False Discovery Rate. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.