Fig. 2: Protein level and cleavage of GSDME are elevated in IMQ-induced psoriasis-like dermatitis. | Cell Death & Disease

Fig. 2: Protein level and cleavage of GSDME are elevated in IMQ-induced psoriasis-like dermatitis.

From: Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation

Fig. 2

A Protocol for inducing psoriasis-like dermatitis in mice using IMQ. B Representative images of dorsal skin from WT mice treated with either Vaseline or IMQ. C Evaluation of PASI scores for two groups from day 0 to day 5. D Day 5 scores for erythema, scaling, thickness, as well as total PASI scores were shown. E Representative H&E-stained images of skin sections. Statistical analysis of epidermal thickness was shown. F GSDME FL protein was presented by immunohistochemistry assay. Statistical analysis on mean staining intensity was shown. G Proteins of GSDME-FL, GSDME-NT, CASP3, and C-CASP3 were detected by western blotting assay. Statistical analysis of interested protein levels was shown. ACTB was used as a loading control. H GSDME FL and C-CASP3 were presented by immunofluorescence assay. Statistical analysis included mean immunofluorescence intensity, colocalization, and Pearson correlation analysis between GSDME FL and C-CASP3. Scale bar is 200 μm in E, F. Scale bar is 100 μm in H. WT wide type, GSDME-FL GSDME full length, GSDME-NT GSDME N-terminal, CAPS3 caspase-3, C-CAPS3 cleaved caspase-3. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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