Fig. 3: GSDME deficiency alleviates IMQ-induced psoriasis-like dermatitis in mice. | Cell Death & Disease

Fig. 3: GSDME deficiency alleviates IMQ-induced psoriasis-like dermatitis in mice.

From: Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation

Fig. 3

A WT and Gsdme−/− mice were treated with IMQ cream. Representative images of dorsal skin lesion were presented. B PASI scores progression of above two groups from day 0 to day 5. C Scores for erythema, scaling, and thickness, as well as PASI scores on day 5. D Representative H&E-stained images of skin sections. Statistical analysis of epidermal thickness was shown. E Immunohistochemistry study showed markers of keratinocyte proliferation (Ki-67 and Keratin-5) and differentiation (Keratin-1, Filaggrin, and Loricrin). Statistical analysis of positive area percentage or mean staining intensity of interested proteins was shown. F, G Neutrophil infiltration was evaluated by detecting Ly6G and MPO through immunohistochemistry study. Statistical analysis of mean staining intensity was shown. H Proteins of GSDME-FL, GSDME-NT, CASP3, and C-CASP3 were detected by western blotting assay. Statistical analysis of interested protein levels was shown. ACTB served as a loading control. Scale bar is 200 μm in D, F, and G. Scale bar is 100 μm in E. WT wide type, GSDME-FL GSDME full length, GSDME-NT GSDME N-terminal, CAPS3 caspase-3, C-CAPS3 cleaved caspase-3. ns not significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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