Fig. 4: GSDME of keratinocyte is involved in IMQ-induced psoriasis-like dermatitis.
A Proteins of GSDME-FL, GSDME-NT, CASP3, and C-CASP3 were detected in epidermis and dermis lysate from IMQ-treated WT mice through western blotting assay, respectively. Statistical analysis of interested protein levels was shown. B The above proteins were detected in epidermis lysate from WT mice after either Vaseline or IMQ treatment by western blotting assay. Statistical results of interested protein levels were shown. C Krt14Cre/+-Gsdmefl/fl mice and their control mice received either Vaseline or IMQ treatment. Day 5 PASI scores were displayed. D Representative H&E-stained images of skin sections showed histological features. Statistical analysis of epidermal thickness was shown. E TUNEL assay was used to detect dead cells. Statistical analysis of dead cell counts was shown. F, G Immunohistochemistry study showed markers of keratinocyte proliferation (Ki-67, Keration-5, and Keration-14) and differentiation (Keration-1, Filaggrin, and Loricrin). Statistical analysis of positive area percentage or mean staining intensity was shown. H Proteins of GSDME-FL, GSDME-NT, CASP3, and C-CASP3 in epidermis lysate of cKO mice were detected by western blotting assay. Statistical analysis of interested protein levels was shown. I GSDME FL and C-CASP3 were detected by immunofluorescence assay. Statistical analysis of mean immunofluorescence intensity, colocalization, and Pearson correlation analysis between GSDME FL and C-CASP3 were shown. Scale bar is 200 μm in D. Scale bar is 100 μm in E–G and I. WT wide type, GSDME-FL GSDME full length, GSDME-NT GSDME N-terminal, CAPS3 caspase-3, C-CAPS3 cleaved caspase-3. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
