Fig. 2: Transcriptional downregulation of Robo4 promotes IR-induced EC migration and pseudo-angiogenesis. | Cell Death & Disease

Fig. 2: Transcriptional downregulation of Robo4 promotes IR-induced EC migration and pseudo-angiogenesis.

From: Endothelial Robo4 suppresses endothelial-to-mesenchymal transition induced by irradiation and improves hematopoietic reconstitution

Fig. 2

A Immunostaining of Robo4 silenced murine ECs exposed to γ-radiation in vitro with VE-Cadherin, PECAM-1, Claudin-5, and TIE-2 mouse antibodies; Scale bars, 20 µm. B shows a representative blot of endothelial-specific protein levels determined using Western blot and their corresponding densitometric quantifications following Robo4 knockdown and IR. β-actin was used as a loading control; data are shown as mean ± SEM; n ≥ 3. C Invitro Matrigel-facilitated angiogenesis of ECs after lentiviral-mediated Robo4 downregulation and IR. Bars representing total tube length and branching points are shown as mean ± SEM; n ≥ 5. D Transwell and wound healing assays measured migration and invasion abilities after Robo4 silencing in ECs with or without IR. The wound closure and migration rate percentage are displayed as the mean ± SEM; n = 3. E Western blotting and RT-qPCR analysis of endothelial adhesion molecules (VCAM-1 and ICAM-1) in ECs transfected with shRobo4 and the relative control in the presence or absence of IR treatment. Results presented as mean ± SEM. *ρ < 0.05; **ρ < 0.01; ***ρ < 0.001; ****ρ < 0.0001).

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