Fig. 6: Analysis of injured Robo4 knockdown ECs on HSC expansion in a coculture system.

A, B Flow cytometry analysis of HSC frequency after coculture with or without Robo4 knockdown and IR-injured ECs. C Flow cytometric analysis of apoptosis induced by injured endothelial monolayer on HSC using Annexin V and 7-AAD. D, F HSC cell cycle analysis by flow cytometry. The effect of endothelial Robo4 silencing on HSC differentiation and maturation after IR injury was also determined. After seven days, results were collected after HSC coculture with normal or injured Robo4-depleted ECs. E, G Flow cytometry analysis of the proportion of granulocytes, megakaryocytes, erythrocytes, and lymphocytes (All data are analyzed as means ± SEM; n = 3, *ρ < 0.05; **ρ < 0.01; ***ρ < 0.001; ****ρ < 0.0001).