Fig. 7: Histological examination shows heart defects recovery after Sildenafil treatment. | Cell Death & Disease

Fig. 7: Histological examination shows heart defects recovery after Sildenafil treatment.

From: Modulation of cAMP/cGMP signaling as prevention of congenital heart defects in Pde2A deficient embryos: a matter of oxidative stress

Fig. 7

A Picture of Sildenafil (SILD) treated Pde2A+/+ and Pde2A−/− E14.5. embryos. Scale bar = 0.5 cm. B Micro-CT picture of the hearts in Sildenafil-treated Pde2A+/+ and Pde2A−/− embryos. C Ratio between total hearts and ventricular volumes relative to embryo volumes obtained by micro-CT analyses of Sildenafil treated or not treated (NT) Pde2A+/+ and Pde2A−/− embryos. At least n = 5 for each genotype/treatment. D Haematoxylin and Eosin staining of transversal sections of Pde2A+/+ and Pde2A−/− Sildenafil treated embryos, the heart is shown. Left and right ventricles (LV, RV), atria (LA, RA), and interventricular septum (IVS) are indicated. Inset shows a magnification of trabeculae and myocardial wall (MW). E Graphs of contralateral axis, trabeculae thickness and trabeculae number, and myocardial wall analyses in treated and not treated samples. At least n = 5 for each condition/genotype. ANOVA two-way was used to compare Pde2A−/− versus the relative Pde2A+/+ in both conditions and Pde2A−/− not treated versus Pde2A−/− Sildenafil samples. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 ****P ≤ 0.0001.

Back to article page