Fig. 4: miR-184 represses pro-oncogenic pathways.
From: miR-184 represses β-catenin and behaves as a skin tumor suppressor
A–D In silico analysis of RNA sequencing of the epidermis of miR-184 wild type (WT) or knockout (KO) newborns. Metascape analysis (A) and gene heatmap (B) highlight main downregulated pathways in miR-184-KO. C Real time PCR analysis performed on RNA lysates of the epidermis of the indicated genotypes to validate the downregulation (left) or upregulation (right) of selected genes. D Immunofluorescent staining of β-catenin performed on skin section of newborn mice of the indicated genotypes. E Quantification of the nuclear signal (e.g., arrows in D) was performed by Image J software in 8 different fields in each of the 3 biological replicates. Each dot represents an average of a single field. All data represents 3 biological replicates. Scale bars were 20 μm. Statistical significance was assessed by t test (*p < 0.05).
