Fig. 6: Caspase-2 limits chaperone-mediated autophagic degradation of GPX4.
a Immunoblot analysis of GPX4 and caspase-2 expression in H1299p53R273H control or CASP2 siRNA cells. Vinculin is shown as the loading control. b Viability of H1299p53R273H cells with control and CASP2 siRNA at 18 h post-treatment with 1 μM erastin, alone or in combination with 17AAG (500 nM). c Immunoblot analysis of GPX4 and caspase-2 expression in H1299p53R273H control or CASP2 siRNA cells following treatment with 17AAG. Vinculin is shown as the loading control. d Schematic diagram summarizing the role of caspase-2 in ferroptosis. Data represented as mean ± s.e.m. from three independent experiments. An unpaired t-test was used to estimate significant differences in b. P-value is indicated with ***P < 0.001.
