Fig. 4: IRF1 is involved in IGF2BP3-regulated cell growth and migration in GC cells.

A The relative mRNA expressions of IRF1, IRF2, IRF9, IFI6, IFIT1, IFIT3, MX1, OAS1 and ISG15 in shNC and shIGF2BP3 MKN-45 cells were investigated by qRT-PCR analysis. B Cell apoptosis of shNC and shIGF2BP3 MKN-45 cells after treated with IFN-γ (50 ng/mL) for 48 h were measured by flow cytometric analysis, respectively. C Gene expressions in STTTCRNTTT_IRF_Q6 set of shNC and shIGF2BP3 MKN-45 cells identified by Ribo-seq were performed with GSEA. D The expressions of IRF1 and IRF2 in shNC and shIGF2BP3 MKN-45 and AGS cells were measured by western blot analysis (up) and quantitatively analyzed (down), respectively. E The expressions of IRF1 in shNC and shIGF2BP3 MKN-45 cells after transfected with siRNA targeting IRF1 (si-IRF1_001) or a negative control siRNA (siNC) for 48 h were measured by western blot analysis, respectively. F Colony formation of shNC and shIGF2BP3 MKN-45 cells after transfected with siIRF1(si-IRF1_001, si-IRF1_002) or siNC for 48 h were recorded (left) and quantitatively analyzed (right), respectively. G Wound healing of shNC and shIGF2BP3 MKN-45 cells after transfected with siIRF1 (si-IRF1_001) or siNC for 48 h were recorded (left) and quantitatively analyzed (right), respectively.