Fig. 2: OTUD4 positively regulates cell proliferation and invasion of GBM cells. | Cell Death & Disease

Fig. 2: OTUD4 positively regulates cell proliferation and invasion of GBM cells.

From: OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway

Fig. 2

A Western blot and qRT-PCR assays were adopted to detect the expression levels of OTUD4 in the control and OTUD4-knockdown LN229 and U87MG cells. B, C MTT assays and plate cloning assays were performed to test the effect of OTUD4 knockdown on cell proliferation. D, E The cell cycle distribution was analyzed in LN229 and U87MG cells by flow cytometry, and western blot assay were used to detect G2/M cell cycle-related proteins. F Transwell assays were carried out in the control and OTUD4-knockdown GBM cells to detect invasion ability. Scale bar, 20 μm. G Wound-healing assay was performed in the control and OTUD4-knockdown cells. H Western blot assay was conducted to detect the expression levels of invasion marker proteins. All data were expressed as the mean ± SD, n = 3. Student’s t-test was performed to analyze significance. *P < 0.05, **P < 0.01, ***P < 0.001.

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