Fig. 3: The 181–300aa of OTUD4 interacts with CDK1. | Cell Death & Disease

Fig. 3: The 181–300aa of OTUD4 interacts with CDK1.

From: OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway

Fig. 3

A GSEA plots depicted enrichment of the cell cycle G2/M checkpoint in the samples with high OTUD4 expression. FDR, false discovery rate; NES, normalized enrichment score. B Co-IP assay were performed to detect interaction between OTUD4 and G2/M cell cycle-related proteins in 293FT cells. C The influence of gradient ectopic expression of OTUD4 on CDK1 was detected by western blot assay. D, E Co-immunoprecipitation assays were performed to detect interaction between OTUD4 and CDK1 proteins in LN229 and U87MG cells. F The proximity ligation (PLA) assay was applied to detect the interaction of Flag-OTUD4 and CDK1/IgG, CDK1 and IgG in GBM cells. Scale bar, 10 μm. G Interaction detection between CDK1 and OTUD4 truncated domains in 293FT cells.

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