Fig. 5: OTUD4 interacts with FGFR1 and stabilizes FGFR1 by deubiquitination. | Cell Death & Disease

Fig. 5: OTUD4 interacts with FGFR1 and stabilizes FGFR1 by deubiquitination.

From: OTUD4 promotes the progression of glioblastoma by deubiquitinating CDK1 and activating MAPK signaling pathway

Fig. 5

A GSEA plot depicted enrichment of the signaling by FGFR1 in disease in the samples with high OTUD4 expression. FDR, false discovery rate; NES, normalized enrichment score. B, C Co-immunoprecipitation assays and the proximity ligation (PLA) assay were performed to detect interaction between OTUD4 and FGFR1. Scale bar, 10 μm. D Western blot analysis of control and OTUD4-knockdown LN229 cells treated with MG132. E Western blot analysis of the turnover of FGFR1. F–I In the presence of MG132, 293FT cells were co-transfected with Flag-OTUD4 or shOTUD4, and HA-ubiquitin (wild type and mutants) plasmids, and the ubiquitination level of FGFR1 was detected by co-IP. J The effects of OTUD4 knockdown and FGFR1 knockdown on CDK1 protein levels were detected. K MYC-CDK1 and shFGFR1 were co-transfected into 293FT cells, and the interaction between CDK1 and cyclinB1 was detected by co-IP.

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