Fig. 2: CircNUP54 knockdown inhibits HCC cell proliferation, migration, and invasion in vitro. | Cell Death & Disease

Fig. 2: CircNUP54 knockdown inhibits HCC cell proliferation, migration, and invasion in vitro.

From: CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA

Fig. 2

A, B qRT-PCR analysis validated the successful knockdown of circNUP54, but not that of NUP54 mRNA, in Huh7 and SNU449 cells transfected with back-splice junction-specific siRNAs. C, D Growth curves based on CCK-8 assays show the proliferation-inhibitory effect of circNUP54 knockdown on Huh7 and SNU449 cells. E–H Effect of circNUP54 silencing on HCC cells evaluated using colony formation assay. I–L The EdU assays of indicated Huh7 and SNU449 cells. Scale bar = 100 μm. M–P Transwell assays show that circNUP54 knockdown attenuated the cell migration and Matrigel invasion abilities of Huh7 and SNU449 cells. Scale bar = 100 μm. Q–T Cell migratory ability was decreased in circNUP54-downregulated HCC cells, as detected using the wound-healing assay. Scale bar = 200 μm. Data presented as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student’s t-test).

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