Fig. 3: CircNUP54 facilitates HCC growth in vivo. | Cell Death & Disease

Fig. 3: CircNUP54 facilitates HCC growth in vivo.

From: CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA

Fig. 3

A Growth curves of subcutaneous tumors in nude mice (n = 6). Lv-sh-NC and Lv-sh-circNUP54 refer to Huh7 cells with negative control and stable circNUP54 knockdown, respectively. B, C Outlook and weight of subcutaneous xenografts dissected from the mice at the endpoint. D, E Representative IHC images of KI67 staining of subcutaneous tumors and the results of quantitative analysis. Left scale bar = 50 μm, right scale bar = 10 μm. F IVIS images of orthotopic xenograft tumor growth from each mouse and ex vivo fluorescence images of isolated livers collected at the endpoint (n = 4). G Quantitative analysis of the liver fluorescence intensity over time. H–N Same type illustrations with A–G, based on Hep3B cells stably transfected with the vector (Lv-vector) or circNUP54 (Lv-circNUP54). Both subcutaneous and orthotopic xenograft tumor models in nude mice suggested that circNUP54 overexpression promoted Hep3B cell growth in vivo. Data presented as means ± SD of three independent experiments. *p < 0.05, ***p < 0.001 (Student’s t-test).

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