Fig. 7: CircNUP54 promotes HCC progression by targeting BIRC3 via HuR. | Cell Death & Disease

Fig. 7: CircNUP54 promotes HCC progression by targeting BIRC3 via HuR.

From: CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA

Fig. 7

A, B Rescue experiments based on colony formation and EdU showed that BIRC3 overexpression reversed the proliferative suppression caused by circNUP54 knockdown in Huh7 cells. C, D Rescue experiments based on colony formation and EdU revealed that BIRC3 knockdown counteracted the proliferative facilitation induced by circNUP54 overexpression in Hep3B cells. Scale bar = 100 μm. E, F Transwell assays revealed that transfection of the BIRC3 upregulation plasmid rescued the suppressive effect of si-circNUP54 on cell migration and invasion in Huh7 cells. G, H Transwell assays revealed that transfection of si-BIRC3 restored the promotive effect of the circNUP54 upregulation plasmid on cell migration and invasion in Hep3B cells. I Schematic diagram illustrates the mechanism of circNUP54 in HCC progression. Scale bar = 100 μm. Data presented as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test).

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