Fig. 1: Tumor-associated microglia/macrophages phenotyping and functional properties.
From: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice
A RT-qPCR from CD11b+ cells isolated from GM and GM/ABX tumoral hemisphere reveals expression of pro-angiogenic genes; B pro-inflammatory genes and (C) anti-inflammatory genes. Gene expression is normalized to the housekeeping gene Gapdh. Data are presented as the mean ± SD n = 3–8 mice pulled from 3 independent experiments. ***p < 0.001; **p < 0.01; *p < 0.05, Student’s t test. D Representative z-projection confocal images of microglia/macrophages cells in the tumoral core area from GM and GM/ABX Cx3cr1+/gfp mice (green) and CD68 (magenta). Hoechst staining (blu) for nuclei visualization. 60x objective (Scale bar: 25 μm). E Scatter dot plots showing quantification of CD68/gfp signals (from Cx3cr1+/gfp mice) expressed as the percentual area occupied in GM (n = 33/9/3 FOV/slices/mice) and GM/ABX (n = 38/9/3 FOV/slices/mice) mice versus total field of view (FOV). Data are presented as the mean ± SD ***p < 0.001, Student’s t test. F Representative fields of GFP fluorescence measurements in slices from GM and GM/ABX Cx3cr1+/gfp mice at minute 0 (left) and after 15 min (right) of ATP. The arrows refer to the tip of the puff pipette. After 5 min of basal motility recordings, ATP is applied for 15 min (Mg-ATP 2 mM, 8 psi, 100 ms). Note the fluorescence increase in the area around the pipette tip only in control slices. Scale bar: 10 µm. G Time course of fluorescence ratio signal (∆F/F0) measured in a ROI of 10 µm radius, centered on the tip of the ATP-containing pipette, from slices of GM and GM/ABX-treated Cx3cr1+/gfp mice. (GM n = 8/4 fields/mice GM/ABX n = 11/4 fields/mice; Data are presented as the mean ± SD ***p < 0.001, Mann–Whitney test at minutes 10 and 15).
