Fig. 2: Vasculature characterization in GM and GM/ABX mice.
From: Antibiotics treatment promotes vasculogenesis in the brain of glioma-bearing mice
A Representative z-projection confocal images of CD34 (magenta) in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 50 μm. B Scatter dot plots showing quantification of CD34 signal expressed as the percentual area occupied by fluorescent CD34 staining in GM (n = 22/5 slices/mice) and GM/ABX (n = 23/5 slices/mice) mice versus total FOV (top) and number of CD34+ vessel-like structure versus total field of view (bottom) GM (n = 22/5 slices/mice) and GM/ABX (n = 23/5 slices/mice). Data are presented as the mean ± SD ***p < 0.001; **p < 0.01, Student’s t test. C Representative z-projection confocal images of CD34 (magenta) and CD31 (green) colocalization in GM and GM/ABX tumor core area. Hoechst staining (blu) for nuclei visualization Scale bar: 20 μm. D Scatter dot plots showing quantification of CD31 signal expressed as the percentual area occupied by fluorescent CD34 staining in GM (n = 18/3 slices/mice) and GM/ABX (n = 18/3 slices/mice) mice versus total field of view (left) and number of CD31+ vessel-like structure versus total field of view (GM n = 18/3 slices/mice; GM/ABX n = 15/3 slices/mice) (right). Data are presented as the mean ± SD *p < 0.05, Student’s t test.
