Fig. 4: Characterization of CD45⁺CD34⁺ cells in glioma tissue.

A Representative z-projection confocal images of CD34 (magenta) and CD45 (green) in the GM tumor core area. Hoechst staining (blu) for nuclei visualization. 60x objective (Scale bar: 25 μm; zoom 20 μm). Yellow stars indicate the CD45⁺CD34⁺ cells. B Flow cytometry quantification of CD45⁺CD34⁺ cells in GM and GM/ABX tumoral hemisphere. C CD45^highCD34⁺ population identifying the peripheral infiltrating hematopoietic endothelial progenitor cells and (D) CD45^intCD34⁺ identifying the resident brain population expressing the CD34 marker (mostly likely microglia/macrophages). n = 5 mice per group. Data are presented as the mean ± SD.