Fig. 4: miR-579-3p overexpression induces senescence features in BRAF-mutant melanoma cells. | Cell Death & Disease

Fig. 4: miR-579-3p overexpression induces senescence features in BRAF-mutant melanoma cells.

From: The MITF/mir-579-3p regulatory axis dictates BRAF-mutated melanoma cell fate in response to MAPK inhibitors

Fig. 4

A M14 and WM266 cells left untreated, treated with Dabrafenib, transfected with miR-579-3p mimic or with SCR sequences have been stained after 72 hours with crystal violet to measure proliferation. B M14 and WM266 cells treated as reported above were fixed and stained for b-galactosidase after 72 hours of the relative treatments. Results have been then quantified counting b-Gal positive cells over the total cells present in ten different fields. C Representative images are reported at original magnification= ×20, the arrows indicate b-Gal positive cells. Student’s t test was performed to determine statistical significance ****p < 0.0001.

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