Fig. 2: The absence of Agrin leads to progressive muscle fiber atrophy, fiber type conversion, and loss of muscle mass. | Cell Death & Disease

Fig. 2: The absence of Agrin leads to progressive muscle fiber atrophy, fiber type conversion, and loss of muscle mass.

From: Deficiency of skeletal muscle Agrin contributes to the pathogenesis of age-related sarcopenia in mice

Fig. 2

A Schematic diagrams of Pax7-Cre-driven deletion of Agrin in mice. B Representative immunofluorescence staining of Agrin (red) in control and cKO TA muscle sections and quantification of data, n = 5 mice per genotype. C Bodyweights of control and cKO mice at different ages. n = 15 mice per genotype. D Muscle weights of control and cKO mice at 3 and 12 months of age. n = 15 mice per group. E Representative H&E staining images of TA muscle (fast twitch) cross-sections. F Quantification of total fiber number and CNF percentage in TA muscles (fast twitch). n = 15 mice per group. G α-Laminin staining showing the relative size of myofibers in TA muscles from mice at 12 months of age. H Frequency of distribution for CSA (µm2) of TA muscle (n = 5 per genotype). I Representative immunofluorescence staining of α-Laminin (red) and MyHC-2B (green) in TA muscle in mice at 12 months of age. J Quantification of data in I, n = 7 mice per genotype. Scale bar = 50 μm, *p < 0.05, **p < 0.01, ****p < 0.0001.

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