Fig. 4: ECT2 mediates the regulation of GINS2 on malignant phenotype and TMZ sensitivity in glioma cells.
From: GINS2 regulates temozolomide chemosensitivity via the EGR1/ECT2 axis in gliomas
A Western blotting to detect the effect of transferring the ECT2 plasmid in GINS2 knockout cells. B Cell proliferation measurement of Cas9 Ctrl, KO-GINS2 + OE-Vector and KO-GINS2 + OE-ECT2 glioma cells. C Clone-formation ability measurement of Cas9 Ctrl, KO-GINS2 + OE-Vector and KO-GINS2 + OE-ECT2 glioma cells. D Stemness measurement of Cas9 Ctrl, KO-GINS2 + OE-Vector and KO-GINS2 + OE-ECT2 glioma cells. Invasion and migration capacity measurement (E, F) of Cas9 Ctrl, KO-GINS2 + OE-Vector and KO-GINS2 + OE-ECT2 glioma cells. NS: No significance. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. n = 3 independent experiments. Two-tailed t-test assuming equal variances. Error bars represent the mean ± standard deviation of the mean.
