Fig. 2: GFPT2 overexpressing pancreatic cancer cells promoted macrophage M2 polarization. | Cell Death & Disease

Fig. 2: GFPT2 overexpressing pancreatic cancer cells promoted macrophage M2 polarization.

From: The GFPT2-O-GlcNAcylation-YBX1 axis promotes IL-18 secretion to regulate the tumor immune microenvironment in pancreatic cancer

Fig. 2

A The pattern diagram showed the coculture system of pancreatic cancer cells and macrophages which was derived from CD14+ monocytes. B KEGG analysis of transcriptome sequencing data on macrophages cocultured with GFPT2-knockdown CFPAC-1 cells. C CFPAC-1, SW1990 and PANC-1 stable cell lines were cocultured with macrophages for 3 days and then M2 macrophage were analyzed by flow cytometry with CD11b, CD45, CD163 and CD206 antibodies. D, E Multiplex immunohistochemistry assay showing the macrophage M2 polarization by CD206 and CD68 antibodies and the proportion of M2 in all macrophages was calculated.

Back to article page