Fig. 2: Identification of Motins as RNF166 interacted protein. | Cell Death & Disease

Fig. 2: Identification of Motins as RNF166 interacted protein.

From: RNF166 promotes colorectal cancer progression by recognizing and destabilizing poly-ADP-ribosylated angiomotins

Fig. 2

A Protein–protein interaction network of RNF166 from the Bioplex interactome database. Interplay between RNF166 and Motins. 293 T cells (B−D) or HCT116 cells (E) transfected with the indicated plasmids. Cell extracts were incubated with streptavidin agarose (B, C, E) or immunoprecipitated with normal mouse IgG/anti-Myc antibody (D). F Orthogonal projections illustrating the colocalization of RNF166 (red) and AMOTL2 (green). Indicated (yellow) intensity trace is plotted at right. HCT116 cells were stably overexpressed Flag-RNF166. Samples were coimmunized with mouse anti-Flag and rabbit anti-AMOTL2 antibodies and incubated with goat anti-mouse and goat anti-rabbit secondary antibodies. DNA was stained with DAPI (blue). Scale bar, 50 μm. Abbreviations: DAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride.

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